Molecular Virology
Oncolytic Herpes Viruses
retargeted to cancer-specific receptors
European Reserch Council - Alma Mater Studiorum università di Bologna


List of our publications since 2001 to date: full papers on international journals with impact factor and book chapters; patents.

Since the beginning of the ERC project, we have published three papers in highly ranking journals and deposited a patent application (highlighted in red).

2015 (# 61 - 63)

63. Leoni V, Gatta V, Palladini A, Nicoletti G, Ranieri D, Dall'Ora M, Grosso V, Rossi M, Alviano F, Bonsi L, Nanni P, Lollini PL, Campadelli-Fiume G. Systemic delivery of HER2-retargeted oncolytic-HSV by mesenchymal stromal cells protects from lung and brain metastases. Oncotarget. 2015 Sep 27. [Epub ahead of print] DOI: 10.18632/oncotarget.5793 PMID: 26430966

62. Gianni T, Massaro R, Campadelli-Fiume G. Dissociation of HSV gL from gH by αvβ6- or αvβ8-integrin promotes gH activation and virus entry. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):E3901-10. doi: 10.1073/pnas.1506846112. Epub 2015 Jul 8. PMID: 26157134

61. Gatta V, Petrovic B, Campadelli-Fiume G. The Engineering of a Novel Ligand in gH Confers to HSV an Expanded Tropism Independent of gD Activation by Its Receptors. PLoS Pathog. 2015 May 21;11(5):e1004907. doi: 10.1371/journal.ppat.1004907. eCollection 2015 May. PMID: 25996983

2011 - 2015 (# 48 - 60)

60. Gianni T, Campadelli-Fiume G. The epithelial αvβ3-integrin boosts the MYD88-dependent TLR2 signaling in response to viral and bacterial components. PLoS Pathog. 2014 Nov 6;10(11):e1004477. doi: 10.1371/journal.ppat.1004477. eCollection 2014 Nov. PMID: 25375272

59. Gianni T, Salvioli S, Chesnokova LS, Hutt-Fletcher LM, Campadelli-Fiume G. αvβ6- and αvβ8-integrins serve as interchangeable receptors for HSV gH/gL to promote endocytosis and activation of membrane fusion. PLoS Pathog. 2013;9(12):e1003806. doi: 10.1371/journal.ppat.1003806. Epub 2013 Dec 19.
PMID: 24367260

58. Gianni T, Leoni V, Campadelli-Fiume G. Type I interferon and NF-κB activation elicited by herpes simplex virus gH/gL via αvβ3 integrin in epithelial and neuronal cell lines. J Virol. 2013 Dec;87(24):13911-6. doi: 10.1128/JVI.01894-13. Epub 2013 Oct 9. PMID: 24109241 

57. Nanni P, Gatta V, Menotti L, De Giovanni C, Ianzano M, Palladini A, Grosso V, Dall’Ora M, Croci S, Nicoletti G, Landuzzi L, Iezzi M, Campadelli-Fiume G, Lollini P-L. Preclinical therapy of disseminated HER-2+ ovarian and breast carcinomas with a HER-2-retargeted oncolytic herpesvirus. PLoS Pathog. 2013 Jan;9(1):e1003155. doi:10.1371/journal.ppat.1003155. Epub 2013 Jan 31. PMID: 23382683

56. Gianni T, Leoni V, Chesnokova LS, Hutt-Fletcher LM, Campadelli-Fiume G. {alpha}V{beta}3-integrin is a major sensor and activator of innate immunity to herpes simplex virus-1. Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19792-7. Epub 2012 Nov 12. PMID: 23150579

55. Reisoli E, Gambini E, Appolloni I, Gatta V, Barilari M, Menotti L, Malatesta P. Efficacy of HER2 retargeted herpes simplex virus as therapy for high-grade glioma in immunocompetent mice. Cancer Gene Ther. 2012 Nov;19(11):788-95. Epub 2012 Sep 21. PMID: 22996742

54. Leoni V, Gianni T, Salvioli S, Campadelli-Fiume G. Herpes simplex virus glycoproteins gH/gL and gB bind Toll-like receptor 2, and soluble gH/gL is sufficient to activate NF-κB. J Virol. 2012 Jun;86(12):6555-62. Epub 2012 Apr 11. PMID: 22496225

53. Campadelli-Fiume G, Menotti L, Avitabile E, Gianni T. Viral and cellular contributions to herpes simplex virus entry into the cell. Curr Opin Virol. 2012 Feb;2(1):28-36. Epub 2012 Jan 4. Review. PMID: 22440963

52. Gambini E, Reisoli E, Appolloni I, Gatta V, Campadelli-Fiume G, Menotti L, Malatesta P. Replication-competent herpes simplex virus retargeted to HER2 as therapy for high-grade glioma. Mol Ther. 2012 May;20(5):994-1001. Epub 2012 Feb 21. PMID: 22354378

51. Campadelli-Fiume G, Menotti L, Zhou G, De Giovanni D, Nanni P and Lollini P-L. Herpesviruses as oncolytic agents. In: Blaho JA and Baines JD, Editors. From the Hallowed Halls of Herpesvirology. A Tribute to Bernard Roizman. World Scientific Publishing Co. Pte. Ltd., Singapore 596224 (ISBN-13 978-981-4338-98-1, ISBN-10 981-4338-98-2); 2012. Chapter 10, p. 223 - 250. Book chapter.

50. Gianni T, Campadelli-Fiume G. {alpha}V{beta}3-integrin relocalizes nectin1 and routes herpes simplex virus to lipid rafts. J Virol. 2012 Mar;86(5):2850-5. Epub 2011 Dec 14. PMID: 22171266

49. Menotti L, Campadelli-Fiume G, Nanni P, Lollini PL, De Giovanni C. The molecular basis of herpesviruses as oncolytic agents. Curr Pharm Biotechnol. 2012 Jul;13(9):1795-803. PMID: 21740356

48. Campadelli-Fiume G, De Giovanni C, Gatta V, Nanni P, Lollini PL, Menotti L. Rethinking herpes simplex virus: the way to oncolytic agents. Rev Med Virol. 2011 Jul;21(4):213-26. Epub 2011 May 27. Review. PMID: 21626603


2006 - 2010 (# 24 - 47)

47. Campadelli-Fiume G. The egress of alphaherpesviruses from the cell. In: Arvin A, Campadelli-Fiume G, Mocarski E, Moore PS, Roizman B, Whitley R, Yamanishi K, editors. Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge: Cambridge University Press; 2007. Chapter 12. PMID: 21348111

46. Campadelli-Fiume G, Menotti L. Entry of alphaherpesviruses into the cell. In: Arvin A, Campadelli-Fiume G, Mocarski E, Moore PS, Roizman B, Whitley R, Yamanishi K, editors. Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge: Cambridge University Press; 2007. Chapter 7. PMID: 21348078

45. Roizman B, Campadelli-Fiume G. Alphaherpes viral genes and their functions. In: Arvin A, Campadelli-Fiume G, Mocarski E, Moore PS, Roizman B, Whitley R, Yamanishi K, editors. Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge: Cambridge University Press; 2007. Chapter 6. PMID: 21348062

44. Gianni T, Gatta V, Campadelli-Fiume G. {alpha}V{beta}3-integrin routes herpes simplex virus to an entry pathway dependent on cholesterol-rich lipid rafts and dynamin2. Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22260-5. Epub 2010 Dec 6. PMID: 21135248

43. Buonaguro FM, Campadelli-Fiume G, De Giuli Morghen C, Palù G. Updates and achievements in virology. Expert Rev Vaccines. 2010 Jul;9(7):703-6. PMID: 20624042

42. Gabev E, Tobler K, Abril C, Hilbe M, Senn C, Franchini M, Campadelli-Fiume G, Fraefel C, Ackermann M. Glycoprotein D of bovine herpesvirus 5 (BoHV-5) confers an extended host range to BoHV-1 but does not contribute to invasion of the brain. J Virol. 2010 Jun;84(11):5583-93. Epub 2010 Mar 10. PMID: 20219909

41. Gianni T, Cerretani A, Dubois R, Salvioli S, Blystone SS, Rey F, Campadelli-Fiume G. Herpes simplex virus glycoproteins H/L bind to cells independently of {alpha}V{beta}3 integrin and inhibit virus entry, and their constitutive expression restricts infection. J Virol. 2010 Apr;84(8):4013-25. Epub 2010 Feb 10. PMID: 20147400

40. Lollini PL, Menotti L, De Giovanni C, Campadelli-Fiume G, Nanni P. Oncolytic herpes virus retargeted to HER-2. Cell Cycle. 2009 Sep 15;8(18):2859-60. Epub 2009 Sep 26. PMID: 19729994

39. Avitabile E, Forghieri C, Campadelli-Fiume G. Cross talk among the glycoproteins involved in herpes simplex virus entry and fusion: the interaction between gB and gH/gL does not necessarily require gD. J Virol. 2009 Oct;83(20):10752-60. Epub 2009 Aug 5. PMID: 19656900

38. Menotti L, Nicoletti G, Gatta V, Croci S, Landuzzi L, De Giovanni C, Nanni P, Lollini PL, Campadelli-Fiume G. Inhibition of human tumor growth in mice by an oncolytic herpes simplex virus designed to target solely HER-2-positive cells. Proc Natl Acad Sci U S A. 2009 Jun 2;106(22):9039-44. Epub 2009 May 20. PMID: 19458262

2001 - 2005 (#1 - 23)

23.       Avitabile E, Lombardi G, Gianni T, Capri M, Campadelli-Fiume G. Coexpression of UL20p and gK inhibits cell-cell fusion mediated by herpes simplex virus glycoproteins gD, gH-gL, and wt- gB or an endocytosis-defective gB mutant, and downmodulates their cell surface expression. J Virol 2004, 78:8015-8025. PMID: 15254173

22.       Cocchi F, Fusco D, Menotti L, Gianni T, Eisenberg RJ, Cohen GH, Campadelli-Fiume G. The soluble ectodomain of herpes simplex virus gD contains a membrane-proximal pro-fusion domain and suffices to mediate virus entry. Proc Natl Acad Sci U S A 2004, 101:7445-7450. PMID: 15123804

21.       Cocchi F, Menotti L, Di Ninni V, Lopez M, Campadelli-Fiume G. The herpes simplex virus JMP mutant enters receptor-negative J cells through a novel pathway independent of the known receptors nectin1, HveA, and nectin2. J Virol 2004, 78:4720-4729. PMID: 15078954

20.       Bertucci C, Cimitan S, Menotti L. Optical biosensor analysis in studying herpes simplex virus glycoprotein D binding to target nectin1 receptor. J Pharm Biomed Anal 2003, 32:697-706. PMID: 12899960

19.       Medici MA, Sciortino MT, Perri D, Amici C, Avitabile E, Ciotti M, Balestrieri E, De Smaele E, Franzoso G, Mastino A. Protection by herpes simplex virus glycoprotein D against Fas-mediated apoptosis: role of nuclear factor kappaB. J Biol Chem 2003, 278:36059-36067. PMID: 12844494

18.       Orlandi M, Mantovani B, Ammar K, Avitabile E, Dal Monte P, Bartolini G. Retinoids and cancer: antitumoral effects of ATRA, 9-cis RA and the new retinoid IIF on the HL-60 leukemic cell line. Med Princ Pract 2003, 12:164-169. PMID: 12766334

17.       Avitabile E, Lombardi G, Campadelli-Fiume G. Herpes simplex virus glycoprotein K, but not its syncytial allele, inhibits cell-cell fusion mediated by the four fusogenic glycoproteins, gD, gB, gH, and gL. J Virol 2003, 77:6836-6844. PMID: 12768003

16.       Zhou G, Avitabile E, Campadelli-Fiume G, Roizman B. The domains of glycoprotein D required to block apoptosis induced by herpes simplex virus 1 are largely distinct from those involved in cell- cell fusion and binding to nectin1. J Virol 2003, 77:3759-3767. PMID: 12610150

15.       Menotti L, Cocchi F, Campadelli-Fiume G. Critical residues in the CC' ridge of the human nectin1 receptor V domain enable herpes simplex virus entry into the cell and act synergistically with the downstream region. Virology 2002, 301:6-12. PMID: 12359441

14.       Fabre S, Reymond N, Cocchi F, Menotti L, Dubreuil P, Campadelli-Fiume G, Lopez M. Prominent role of the Ig-like V domain in nectins' trans-interactions: Nectin3 and nectin4 bind to the C-C'-C" beta-strands of the nectin1 V domain. J Biol Chem 2002, 14:14. PMID: 12011057

13.       Menotti L, Casadio R, Bertucci C, Lopez M, Campadelli-Fiume G. Substitution in the murine nectin1 receptor of a single conserved amino acid at a position distal from herpes simplex virus gD binding site confers high affinity binding to gD. J Virol 2002, 76:5463-5471. PMID: 11991974

12.       Cocchi F, Lopez M, Dubreuil P, Campadelli-Fiume G, Menotti L: Chimeric nectin1-poliovirus receptor molecules identify a nectin1 region functional in herpes simplex virus entry. J Virol 2001, 75:7987-7994. PMID: 11483743

11.       Lopez M, Cocchi F, Avitabile E, Leclerc A, Adelaide J, Campadelli-Fiume G, Dubreuil P. Novel, soluble isoform of the herpes simplex virus (HSV) receptor nectin1 (or PRR1-HIgR-HveC) modulates positively and negatively susceptibility to HSV infection. J Virol 2001, 75:5684-5691. PMID: 11356977

10.       Menotti L, Avitabile E, Dubreuil P, Lopez M, Campadelli-Fiume G. Comparison of murine and human nectin1 binding to herpes simplex virus glycoprotein D (gD) reveals a weak interaction of murine nectin1 to gD and a gD-dependent pathway of entry. Virology 2001, 282:256-266. PMID: 11289808

9.         Zhou G, Galvan V, Campadelli-Fiume G, Roizman B: Glycoprotein D or J delivered in trans blocks apoptosis in SK-N-SH cells induced by a herpes simplex virus 1 mutant lacking intact genes expressing both glycoproteins. J Virol 2000, 74:11782-11791. PMID: 11090178

8.         Campadelli-Fiume G: Virus receptor arrays, CD46 and human herpesvirus 6. Trends Microbiol 2000, 8:436-438. PMID: 11044670

7.         Reymond N, Borg J, Lecocq E, Adelaide J, Campadelli-Fiume G, Dubreuil P, Lopez M. Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin. Gene 2000, 255:347-355. PMID: 11024295

6.         Campadelli-Fiume G, Cocchi F, Menotti L, Lopez M. The novel receptors that mediate the entry of herpes simplex viruses and animal alphaherpesviruses into cells. Rev Med Virol 2000, 10:305-319. PMID: 11015742

5.         Kempf W, Adams VV, Burg G, Panizzon RG, Campadelli-Fiume G, Nestle FO. Is pityriasis rosea skin healthier than healthy skin? Arch Dermatol 2000, 136:932-934. PMID: 10891001

4.         Menotti L, Lopez M, Avitabile E, Stefan A, Cocchi F, Adelaide J, Lecocq E, Dubreuil P, Campadelli Fiume G. The murine homolog of human-Nectin1d serves as a species non-specific mediator for entry of human and animal aherpesviruses in a pathway independent of a detectable binding to gD. Proc Natl Acad Sci U S A 2000, 97:4867-4872. PMID: 10781093

3.         Kempf W, Muller B, Maurer R, Adams V, Campadelli Fiume G. Increased expression of human herpesvirus 7 in lymphoid organs of AIDS patients. J Clin Virol 2000, 16:193-201. PMID: 10738138

2.         Cocchi F, Menotti L, Dubreuil P, Lopez M, Campadelli Fiume G. Cell-to-cell spread of wild type herpes simplex virus 1, but not of syncytial strains, is mediated by the immunoglobulin-like receptors that mediate virion entry, nectin1 (HveC/HIgR/PRR1) and nectin2 (PRR2). J Virol 2000, 74:3909-3917. PMID: 10729168

1.         Lopez M, Cocchi F, Menotti L, Avitabile E, Dubreuil P, Campadelli-Fiume G. Nectin2a (PRR2a or HveB) and nectin2d are low-efficiency mediators for entry of herpes simplex virus mutants carrying the Leu25Pro substitution in glycoprotein D. J Virol 2000, 74:1267-1274. PMID: 10627537

  • Campadelli-Fiume G, Gatta V. "Retargeted HSV using scFv-gH fusion" Alma Mater Studiorum Università di Bologna - Filling date: February 11, 2015. European Patent Application No. 15 425 012.0
  • Secchiero P and Campadelli-Fiume G. Human herpesvirus-7 diagnostic and immunologic reagents and methods therefor. PCT Patent application PCT/US98/14372. Joint Patent University of Bologna and UMBI University of Maryland.
  • Campadelli-Fiume G, Cocchi F, Menotti L, and Lopez M. HIgR and related V domain for the manufacture of a medicament for preventing or treating HSV-1, HSV-2 and BHV infections. European Patent Office Application nº 98830677.5-5-2105. Depositato il 10-11-1998 in Germania per Brevetto "Europeo" in Europa, USA (brevetto accordato), Giappone. Assegnatari: Università di Bologna (Italia), INSERM (Francia).
  • Campadelli-Fiume G, Menotti L. Modificazione del tropismo del virus herpes simplex verso recettori selezionati. 2005. Language: Italian. Property: Alma Mater Studiorum - Università di Bologna. Number: BO2005A000788
  • Campadelli-Fiume G, Menotti L. Herpes simplex virus (HSV) with modified tropism, uses and process of preparation thereof. PCT/IT2008/000358. Request deposited 29-5-2008.